TEMPERATURE-DEPENDENCE OF ADRIAMYCIN, CIS-DIAMMINEDICHLOROPLATINUM, BLEOMYCIN, AND 1,3-BIS(2-CHLOROETHYL)-1-NITROSOUREA CYTO-TOXICITY INVITRO

  • 1 January 1983
    • journal article
    • research article
    • Vol. 43  (2) , 517-520
Abstract
The effect of mildly hypothermic temperatures (22.degree.-32.degree. C) on the cytotoxicity of adriamycin, cis-diamminedichloroplatinum, bleomycin, and 1,3-bis(2-chloroethyl)-1-nitrosourea was examined in Chinese hamster ovary cells in vitro. Over a dose range of adriamycin, cell killing at 30.degree. C was reduced by 1-3 orders of magnitude as compared to that at 37.degree. C. cis-Diammminedichloroplatinum was also less cytotoxic at 30.degree. C (0.4-1.2 orders of magnitude) than at 37.degree. C. For bleomycin and 1,3-bis(2-chloroethyl)-1-nitrosourea, the reduction in cytotoxicity at 30.degree. C in comparison to 37.degree. C was less marked. All drugs were more toxic at 42.4.degree.-43.degree. C than at 37.degree. C. Precooling of cells for 2 h at 30.degree. C did not alter the cell killing caused by these drugs at elevated temperatures. A more selective anticancer effect might result if some chemotherapeutic drugs were administered during whole-body hypothermia and regional-local hyperthermia of tumor masses.