Isolation and sequencing of porcine fast skeletal muscle alkali myosin light chain 3 cDNA
- 1 October 1997
- journal article
- research article
- Published by Taylor & Francis in Animal Biotechnology
- Vol. 8 (2) , 179-185
- https://doi.org/10.1080/10495399709525880
Abstract
A cDNA coding for alkali myosin light chain 3 (MLC3F) was isolated from a porcine skeletal muscle library. This clone has an insert of 859 bp encompassing the complete CDS (coding sequence) plus the 5’ and 3’ untranslated regions. Computer analysis showed that porcine MLC3F cDNA is highly homologous to the corresponding cDNAs of human, rabbit and rat. Moreover, Northern analysis showed the presence of two bands that represent the mature mRNAs of the MLClF and MLC3F isoforms according to data observed in other species.Keywords
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