Development of Latent Infections on Cranberry Leaves Inoculated withBotryosphaeria vaccinii

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Abstract
The range of dew period temperatures conducive to the establishment of latent leaf infections and the histopathological relationship of B. vaccinii to cranberry (V. macrocarpon) leaves were investigated in growth chamber tests with inoculated cuttings. Inoculated plants were placed in dew chambers at 16, 20, 24, 28 or 32.degree. C for 72 h then incubated at 20 or 28.degree. C for 6 wk. Latent infections were established most often with a 28.degree. C dew period followed by a 28.degree. C incubation. Microscopic examination of inoculated leaves demonstrated conidial germination and appressorial development within 24 h. Appressoria usually formed adjacent to germinating spores without prior germ tube elongation. Penetration pegs developed within 48-72 h after inoculation followed by the formation of dormant infection hyphae within the cuticle or between the cuticle and outer wall of the epidermis. No additional development was noted up to 2 wk after inoculation.

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