Occurrence of two distinct citrate synthases in a mutant of Pseudomonas aeruginosa and their growth‐dependent variation

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Abstract
Two distinct citrate synthases have been found in a mutant of Pseudomonas aeruginosa — a ‘large’ form (M r 2̃50 000) and a ‘small’ form (M r 1̃00 000). In common with the citrate synthase of wild‐type Ps. aeruginosa and other aerobic Gram‐negative bacteria, the ‘large’ enzyme is regulated by both NADH and AMP, whereas the ‘small’ enzyme is insensitive to these effectors. The relative proportions of the two citrate synthases vary with the stage of growth of the bacterial culture. The ‘large’ form predominates in logarithmic phase, whereas the ‘small’ form is the major component in stationary phase. The two forms do not appear to be interconvertible by a simple dissociation—association mechanism.