Proteoglycans of human gingival epithelium and connective tissue

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Abstract
Proteoglycans extracted from separated specimens of healthy human gingival epithelium and from connective tissue were purified. Epithelial proteoglycans fractionated as a single included peak on Sepharose 4B-CL and contained heparan sulfate and dermatan sulfate glycosaminoglycans. Connective tissue proteoglycans separated into 3 major populations on Sepharose 4B-CL, one of which was excluded from this gel under associative conditions (0.5 M sodium acetate, pH 7.4). Subsequent fractionation of the excluded material under dissociative conditions (4 M guanidinium chloride/0.05 M sodium acetate, pH 7.4) revealed an absence of any aggregate formation of molecules within this population. Connective tissue proteoglycans contained heparan sulfate, dermatan sulfate and chondroitin 4-sulfate, the proportions of which varied with the molecular size of the proteoglycans. Amino acid analysis of the protein cores of gingival-epithelial and connective-tissue proteoglycans revealed differences that were similar to the differences described between other types of proteoglycans such as those from skin.