ORTHOTOPIC RAT LIVER TRANSPLANTATION AFTER LONG-TERM PRESERVATION BY CONTINUOUS PERFUSION WITH FLUOROCARBON EMULSION

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Abstract
Isolated rat livers were stored for up to 25 h using a continuous pulsatile perfusion technique with and without fluorocarbon emulsion oxygen carrier in the perfusate. After preservation, orthotopically isografted livers can maintain recipient animals in good health. The longest surviving animal is alive at 12 mo. A prosthetic cuff technique for the portal vein anastomosis was specially developed for this experimental series. It has simplified vascular anastomosis after the portal vein cannulation that is required for mechanical preservation of the donor liver. The described method in the rat liver isograft model is apparently useful for studies of liver preservation.