A Rapid Silver Impregnation Method for Nervous Tissue: A Modified Protargol-Peroxide Technic

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Abstract
A rapid, reliable Ag impregnation method is described for nervous tissue fixed in formol-saline, Bouin or Susa. Sections are impregnated for 10-15 min at room temperature or 37.degree. C in a solution containing 0.5 g Protargol-S, 0.005-0.01 g allantoin, 1 ml of 1% Cu[No3]2, 1 ml of 1% AgNO3 and 1-2 drops of 30% H2O2 in 100 ml distilled water. Thereafter the sections are reduced in a hydroquinone-formalin solution. This is followed by Au toning and subsequent reduction and mounting. Alternatively, following the first reduction, the Ag image can be intensified by placing sections in a silver-allantoin bath which is followed by reduction and mounting. This method is very reliable and selective, making it suitable for general routine and research use.