Product and product-independent induction of butane oxidation inPseudomonas butanovora
Open Access
- 1 September 2005
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 250 (1) , 111-116
- https://doi.org/10.1016/j.femsle.2005.06.058
Abstract
Pseudomonas butanovora grows on butane by means of an inducible soluble alkane monooxygenase (sBMO). The induction of sBMO was studied using the wild type and a sBMO reporter strain. The reporter strain has the lacZ::kan cassette inserted into bmoX, the gene that encodes the α-subunit of the hydroxylase of sBMO. The β-galactosidase activity in the reporter strain was not induced by butane, but was induced by 1-butanol and butyraldehyde. P. butanovora expressed sBMO product-independent activity at 3.0 ± 1 nmol ethylene oxide min−1 mg protein−1 in stationary phase. The sBMO product-independent activity likely primes the expression of sBMO by butane.Keywords
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