Flow Cell CCC/FT-IR Spectrometry

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Abstract
The development of flow cell LC/FT-IR spectrometry has been plagued by the fact that liquid chromatographic mobile phases absorb infrared radiation. This problem is exacerbated by low solute-to-solvent ratios in the eluates. High speed countercurrent chromatography (CCC) is used in this study to obtain high solute-to-solvent ratios that alleviate some of the problems of infrared absorption of the mobile phase. The high solute-to-solvent ratios allow the use of a simple flow cell interface which makes complex solvent removal procedures unnecessary. CCC/FT-IR (Countercurrent chromatography/Fourier transform infrared) spectrometry is useful for applications where the analyst is not limited by sample size, and high sample loadings are possible. CCC/FT-IR spectrometry is particularly useful to users of countercurrent chromatography to obtain useful structural information of mixture components with an FT-IR spectrometer.