New Techniques for the Rapid Characterization of Oligonucleotides by Mass Spectrometry
- 1 June 1999
- journal article
- research article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 18 (6-7) , 1549-1553
- https://doi.org/10.1080/07328319908044782
Abstract
Recent advances in combined HPLC/electrospray ionization-mass spectrometry provide effective new capabilities for the rapid characterization of oligonucleotides. Accurate mass measurements with errors <0.3 Da, and determination of base and sugar modification and of nearest neighbor identities, can be routinely carried out on 10-100 component mixtures of RNA or DNA. These procedures are widely applicable in structural and analytical studies involving mixtures of oligonucleotides.Keywords
This publication has 8 references indexed in Scilit:
- Detection and Structure Analysis of Modified Nucleosides in RNA by Mass SpectrometryPublished by American Society for Microbiology ,2014
- Presence and location of modified nucleotides in Escherichia coli tmRNA: structural mimicry with tRNA acceptor branchesThe EMBO Journal, 1998
- Applications of mass spectrometry to the characterization of oligonucleotides and nucleic acidsCurrent Opinion in Biotechnology, 1998
- Analysis of Oligonucleotides by HPLC−Electrospray Ionization Mass SpectrometryAnalytical Chemistry, 1997
- Mass spectrometry of nucleic acidsMass Spectrometry Reviews, 1996
- Indirect mass spectrometric methods for characterizing and sequencing oligonucleotidesMass Spectrometry Reviews, 1996
- Electrospray Ionization for Mass Spectrometry of Large BiomoleculesScience, 1989
- Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltonsAnalytical Chemistry, 1988