Myxosoma cerebralis: Trypsinization of Plankton Centrifuge Harvests Increases Optical Clarity and Spore Concentration
- 1 December 1980
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Fisheries and Aquatic Sciences
- Vol. 37 (12) , 2225-2227
- https://doi.org/10.1139/f80-266
Abstract
Pepsin, pronase, and trypsin were compared for their ability to digest the tissue residues that constitute the overwhelming mass of plankton centrifuge harvests. Trypsin was preferred; a treatment with 0.25% for 30 min at pH 7.2–7.5 released an average of 22% additional spores and resulted in an average 11-fold increase in spore concentration. The procedure significantly enhances the sensitivity of spore detection by the plankton centrifuge method.Key words: Myxosoma cerebralis, myxosporidans, spore detectionKeywords
This publication has 2 references indexed in Scilit:
- Myxosoma cerebralis: Fluorescent Antibody Techniques for Antigen RecognitionJournal of the Fisheries Research Board of Canada, 1978
- An improved method for the isolation and quantitative cultivation of embryonic cellsExperimental Cell Research, 1959