Phenyl isocyanate protein compounds and their immunological properties

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Abstract
Phenyl-ureido- and p-bromophenylureido-gelatin were prepared by the method previously descr. These gelatin preparations give marked precipitin and complement fixation reactions with antisera to phenylureido-horse-serum-globulin. With the gelatin preparations the zone of max. precipitation is found in higher dilutions than are the corresponding zones for phenylureido-compounds of horse-serum-globulin or of caseinogen. This difference does not appear to be related to the number of new groupings introduced. The precipitin reactions with these gelatin compounds are most pronounced when carried out at room temp. (15-20[degree]) or at lower temps., since there is a strong tendency for the precipitates to go into solution at 37[degree]. Phenylureido- and p-bromophenylureido-gelatin when injected into rabbits over a long period fail to produce antibodies which are detectable by precipitin and complement fixation tests. Hence the non-antigenicity of gelatin is not due solely to a deficiency in aromatic groupings.