Minor products from the reaction of (+) and (−) benzo[a]-pyrene-anti-diolepoxide with DNA

Abstract
The reaction of [the carcinogen] trans-7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BP-diolepoxide) with deoxyguanosine was studied. In addition to the expected N2-guanine derivative minor products resulting from reaction at the O6 and 7-positions were identified. Reaction of racemic, (+) or (-) BP-diolepoxide with [14C] and [3H]purine labeled DNA allowed same products to be identified and their yields estimated. The O6 and 7-guanine products were apparently derived mainly from reaction of the (-)isomer. The 7-substituted guanine derivative in DNA was unstable, undergoing spontaneous release of the substituted guanine or imidazole ring opening.

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