Immunoelectrophoresis of Duck Sera and Immunoglobulins

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Abstract
Duck whole sera (DWS) and Ig (DIg) were studied in immunoelectrophoresis (IE) and immunodiffusion (ID) tests by rabbit (R) anti-DWS and R anti-DIg sera, and by R antisera monospecific for DIgM and DIgG classes. Immunoelectrophoresis of adult DWS against R anti-DWS illustrated the presence of DIgM and 2 subclasses of DIgG. These observations were verified by the use of monospecific R anti-DIgM and R anti-DIgG sera. DIgM apparently is an electrophoretically heterogeneous protein with components that migrate more slowly than IgM of other species. The cathodal tip of the DIgM extended into the .gamma.2 migration zone almost as far as the DIgG arc. Changes in the IE pattern of DWS from 1- to 14-day-old ducklings were observed that reflected a reduction in DIgG content from the age of 1 to 7 days followed by a gradual change toward the IE appearance of adult sera. Immunoelectrophoresis and ID failed to detect DIgM in sera of newly hatched ducklings. This class of DIg appeared in sera of 7-day-old ducklings and seemed to be more concentrated in sera of 14-day-old ducklings.

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