Inert Site in a Protein Zinc Cluster: Isotope Exchange by High Resolution Mass Spectrometry
- 22 February 2003
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 125 (11) , 3226-3227
- https://doi.org/10.1021/ja0284409
Abstract
Using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry, we show that one Zn2+ ion in the Zn4 cluster of cyanobacterial metallothionein is inert to exchange with 67Zn2+. We suggest that this is zinc in site A, which together with the surrounding α and β secondary structure forms a zinc-finger fold.Keywords
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