CELLULAR EVALUATION OF HYPERSENSITIVITY TO 2,4-DINITROCHLOROBENZENE

  • 1 January 1977
    • journal article
    • research article
    • Vol. 24  (5) , 529-532
Abstract
A suitable modification of the skin window method makes it possible to evaluate hypersensitivity reaction to 2,4-dinitrochlorobenzene at the cellular level. Probands are sensitized by epicutaneous application of 0.2 ml of 1% solution of the tested substance, and after 14 days 1 drop of this solution is applied in the same manner on a freshly scraped area of the skin window. An imprint preparation is taken 48 h later and examined microscopically. Presence of lymphoid elements, eosinophils and basophils suggests a normally developed hypersensitivity reaction. Presence at the same time of rather great amounts of large monocytogenic macrophages and neutrophilic granulocytes is an expression of an unspecific inflammatory reaction taking a normal course. Suitability of the described procedure for routine application is discussed in cancer patients.

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