Identification of the d-glucosides of stigmasterol, sitosterol and campesterol in tobacco and cigarette smoke

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Abstract
A sterol glycoside was isolated from tobacco and cigarette smoke which on acid hydrolysis yielded D -glucose and a sterol mixture. Quantitative determination of the sugar in the aqueous hydrolysate indicated that the isolated material was a monoglucoside. The sterol mixture consisted of stigmasterol, sitosterol and campesterol. Gas-chromatographic analysis, infrared absorption-spectra determination and catalytic hydrogenation established the amounts of stigmasterol, sitosterol, and campesterol in the mixture as 60,25, and 15% respectively.

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