Changes in intracellular calcium and in membrane currents evoked by injection of inositol trisphosphate into Xenopus oocytes
- 22 August 1986
- journal article
- Published by The Royal Society in Proceedings of the Royal Society of London. B. Biological Sciences
- Vol. 228 (1252) , 307-316
- https://doi.org/10.1098/rspb.1986.0057
Abstract
Intracellular calcium was monitored by the use of aequorin in voltage-clamped oocytes of Xenopus laevis . Injection of inositol trisphosphate (IP 3 ) into oocytes elicited slowly rising and decaying aequorin/calcium signals and produced oscillatory chloride membrane currents. These responses did not depend upon extracellular calcium, since they could be elicited in calcium-free solution and after addition of cobalt or lanthanum to block calcium channels in the surface membrane. We conclude that IP 3 causes the release of calcium from intracellular stores in the oocyte. Injections of calcium gave aequorin and membrane current responses that were more transient than those seen with IP 3 .Keywords
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