RESTRICTION ENZYME ANALYSIS OF DNA FROM SPECIES OF BULINUS (BASOMMATOPHORA: PLANORBIDAE) USING A CLONED RIBOSOMAL RNA GENE PROBE

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Abstract
A ribosomal RNA gene probe (pSM889) has been used to study restriction enzyme digests of various species of Bulinus. In order to minimise problems of DNA shearing associated with snail tissues a method of extracting nucleic acids from material embedded in agarose blocks has been used. Restriction enzyme digests with Bgl II and Bam HI hybridised to pSM889 showed clear differences between B. truncatus, B. wrighti, B. africanus and B. forskalii, representing the four species groups of Bulinus. No differences were observed between samples of B. tropicus and B. truncatus digested with Bam HI, Bgl II and Pst I. Intra-specific variation was observed between samples of B. forskalii from Säo Tomé and Angola digested with Bgl II and Hind III although restriction profiles for Bam HI, Pst I and Bst EII digests were similar. Intra-specific variation was also observed between two different population samples of B. wrighti from South Yemen using Bam HI and Bgl II digested genomic DNA hybridised to pSM889.

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