Development of a chromatographic process for largescale purification of staphylococcal enterotoxin B

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Abstract
A process for large-scale purification of staphylococcal enterotoxin B (SEB) is presented. The process consists of three chromatographic steps. The first two steps are based on ion-exchange chromatography and the final one on gel filtration. Diluted culture supernatant (2000 dm3) with a SEB content of 0.4 mg dm−3 were processed and purified. SEB was obtained in 1.5 dm3 of 0.1 mol dm−3 ammonium hydrogen carbonate buffer, pH 8.0. The final product produced a single band on SDS polyacrylamide gel electrophoresis and a single peak when subjected to analytical gel filtration. The overall recovery was 74%.