Production ofβ-Phenethyl Alcohol from Styrene byPseudomonas305-STR-1-4

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Abstract
The productivity of β-phenethyl alcohol (β-PA) from styrene by Pseudomonas 305–STR-1–4 was examined under various conditions. Under the optimum condition, 3.27 mg/ml of β-PA was produced from 20 mg/ml of styrene. The metabolic inhibitor, pyrazole, aided in effectively producing β-PA more reliably and promptly. β-PA was extracted from the culture broth and purified to a semi-viscous, light-yellow liquid with a rose-like fragrance. Recovery was 90% and purity was 99.4%. IR spectrum of this liquid was identical with that of authentic sample. The mechanism of β-PA production by strain 305–STR-1–4 was proposed based on the results of this experiment.