Oral toxicities of Clostridium botulinum type C and D toxins of different molecular sizes

Publisher Website
Google Scholar
Abstract
C. botulinum type C progenitor toxins of different molecular sizes, C-L (16S) and C-M (12S), were purified from cultures of strains 573, Stockholm and CB-19. C-L toxin showed some hemagglutinin activity, whereas C-M toxin did not. Neither C-L nor C-M toxin was activated upon trypsinization. Molecular dissociation of purified type C-L and C-M toxins into toxic and non-toxic components was demonstrated by sucrose density gradient ultracentrifugation and DEAE-Sephadex chromatography at pH 8.0. The molecular construction of type C progenitor toxin may be analogous to that reported for botulinum toxins of other types. C-L and D-L toxins showed higher oral toxicities to mice than did C-M or D-M toxin. Such higher oral toxicities were ascribed to the higher stabilities of these toxins in gastric and intestinal jucies.