Tissue Typing of Cells in Culture. III. HLA Antigens of Established Human Cell Lines. Attempts at Typing by the Mixed Hemadsorption Technique

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Abstract
Established cell lines (30) from solid human tumors were typed by the mixed hemadsorption technique with respect to the HLA-A and HLA-B and partly also the HLA-C series. All cultures exhibited unique antigenic patterns. The discriminatory potential was further increased when taking into consideration one as yet undefined antigen series tentatively named Ek-1 to Ek-11. Sixteen cell lines not designated as HeLA cells but on various non-immunological indications suspected to be HeLa cell contaminants, all gave the same antigenic composition as HeLa cells or clones thereof. The mixed hemadsorption reaction is a highly sensitive and convenient method for immunological typing of cells in monolayer cultures. Some important implications of these results were discussed.
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