SCREENING FOR HIGH-LYSINE CULTIVARS IN A BARLEY BREEDING PROGRAM
- 1 October 1976
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Plant Science
- Vol. 56 (4) , 817-821
- https://doi.org/10.4141/cjps76-133
Abstract
The UDY dye-binding method for determining protein levels in cereal grains was applied to an F2 population derived from a cross between OR 585, a two-rowed selection of barley (Hordeum distichum L. emend Lam.) possessing the "Hiproly" gene(s) for high lysine and TR 412, a two-rowed selection with a normal level of lysine. Seed of the two parental gentoypes produce different UDY absorbance values at similar protein levels. If UDY absorbance values are determined for the parental genotypes over a range of barley protein contents, the different regression lines for OR 585 and TR 412 parental barleys can be used to segregate F2 progeny that express the gene for high lysine. Determination of lysine by the UDY method closely agrees with lysine values determined by amino acid analysis.Keywords
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