New Zealand sheep with scrapie-susceptible PrP genotypes succumb to experimental challenge with a sheep-passaged scrapie isolate (SSBP/1)
- 1 May 2002
- journal article
- Published by Microbiology Society in Journal of General Virology
- Vol. 83 (5) , 1247-1250
- https://doi.org/10.1099/0022-1317-83-5-1247
Abstract
Scrapie does not occur in New Zealand (NZ), although PrP gene alleles associated with susceptibility to the disease are found at relatively high frequencies in NZ sheep. The hypothesis that scrapie is a genetic disease of sheep is thus unlikely to be true. To confirm that NZ sheep are actually susceptible to scrapie infection, NZ sheep of various PrP genotypes were challenged by subcutaneous inoculation with a sheep-passaged scrapie isolate (SSBP/1). Showing similar PrP genetics to that seen in UK sheep, all NZ sheep carrying the VRQ PrP allele developed clinical signs typical of scrapie, with characteristic neurodegenerative changes and PrPSc evident on histopathological examination of their brains and lymphoid tissues. The incubation periods recorded in NZ sheep were generally shorter than those found in UK sheep. The results confirm that New Zealand sheep are as susceptible as their UK counterparts to experimental scrapie infection by subcutaneous inoculation.Keywords
This publication has 20 references indexed in Scilit:
- Early accumulation of PrPSc in gut-associated lymphoid and nervous tissues of susceptible sheep from a Romanov flock with natural scrapieJournal of General Virology, 2000
- Scrapie-free Merino and Poll Dorset sheep from Australia and New Zealand have normal frequencies of scrapie-susceptible PrP genotypes.Journal of General Virology, 1998
- PrP genetics in sheep and the implications for scrapie and BSETrends in Microbiology, 1997
- Immunolocalisation of the prion protein (PrP) in the brains of sheep with scrapieVeterinary Record, 1996
- Identification of five allelic variants of the sheep PrP gene and their association with natural scrapieJournal of General Virology, 1995
- PrP Genotype and Agent Effects in Scrapie: Change In Allelic Interaction With Different Isolates of Agent in Sheep, a Natural Host of ScrapieJournal of General Virology, 1994
- Hydrated autoclave pretreatment enhancement of prion protein immunoreactivity in formalin-fixed bovine spongiform encephalopathy-affected brainActa Neuropathologica, 1994
- Swaledale sheep affected by natural scrapie differ significantly in PrP genotype frequencies from healthy sheep and those selected for reduced incidence of scrapieJournal of General Virology, 1993
- Different scrapie-associated fibril proteins (PrP) are encoded by lines of sheep selected for different alleles of the Sip geneJournal of General Virology, 1991
- Some factors controlling the incidence of scrapie in Cheviot sheep injected with a Cheviot-passaged scrapie agentJournal of Comparative Pathology, 1968