Preparing Bone Sections for Radioautography

Abstract
The method eliminates all contact of bone with aqueous solutions. One hundred to 200 [mu] sections are first cut with a guided circular saw. These sections are simultaneously embedded and mounted on glass slides by means of a clear thermoplastic cement, Gelva. The mounted bone sections are then ground to 5-25[mu] on fine silicon carbide paper. The sections are finally polished with levigated alumina on a cloth pad. A procedure for preparing contact radioautograms of ground bone sections is given and the results obtained are illustrated.

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