A Simple Method for Controlled Freezing of Human Donor Corneae
- 1 January 1986
- journal article
- research article
- Published by S. Karger AG in Ophthalmic Research
- Vol. 18 (6) , 327-331
- https://doi.org/10.1159/000265458
Abstract
A simple method for freezing cultured human donor material with dimethyl sulfoxide (DMSO) was developed empirically in a commercially available microcomputer-controlled apparatus allowing reproducible freezing curves to be made. The optimum cooling rate was found to be 1.0 °C/min down to –40 °C and then 5.0 °C/min to –80 °C. Total cell loss rates were 36.9 ± (SD) 19.8% of postmortem endothelial cell counts. An improved method for evaluating endothelial viability was applied, which enabled us to observe definite cell survival after a postculture period.Keywords
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