Open Gradient Diver Respirometry Modified for Terrestrial Microarthropods

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Abstract
A modification of the open gradient diver method for respirometry developed by Andersen Nexo, Hamburger and Zeuthen is described and discussed. The calculation of O2 consumption is based on recording the downwards migration of ampulla divers in an open linear density gradient made from sodium sulfate solution. Control divers are used for correction of the diver movements caused by fluctuations in temperature and atmospheric pressure. The method is evaluated for accuracy, the influence of experimental conditions on metabolic activity and required investments of equipment and time. The method is a relatively cheap, easy and time-saving alternative to the Cartesian diver technique while retaining a reasonably high sensitivity.