THE DEVELOPMENT OF PEPTONE TOXICITY FOR BRUCELLAE WITH AGING AND THE CORRELATION OF THIS TOXICITY WITH THE PROBABLE OXIDATION OF CYSTINE

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Abstract
A bottle of tryptose (Difco lot 388626) has been tested at intervals since Aug., 1947, for suitability for culturing Brucella abortus 1257. Using 0.1 ml. decimal dilution inocula (through 10-9) from a 48-hr. broth culture of the test organism, this tryptose gave good growth from all inocula in 1947, but subsequently had developed toxicity for all but the 10-1 or 10-2 inocula. A 2d, previously unopened, bottle of tryptose of the same lot number is similarly toxic at the present time. Attempts to correlate this tryptose toxicity with the cystine content of the tryptose have indicated that cystine per se is not toxic for these organisms. However, a heat-induced, breakdown (probably oxidation) product(s) of cystine is toxic for brucellae, and this toxicity appears to be identical with that of toxic tryptose.

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