Isolation and properties of beta-glucosidase from Ruminococcus albus
- 1 January 1985
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 161 (1) , 432-4
- https://doi.org/10.1128/jb.161.1.432-434.1985
Abstract
An enzyme active against p-nitrophenyl-beta-D-glucoside was purified from logarithmic-phase cells of Ruminococcus albus cultivated in a medium containing ball-milled cellulose. The purification yielded homogeneous enzyme after an approximately 520-fold increase in specific activity and a 9% yield. The enzyme was identified as a beta-glucosidase because it can hydrolyze cellobiose and cellooligosaccharides to glucose from the nonreducing ends.Keywords
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