Bioassay for Thrombopoietin Utilizing Mice in Rebound Thrombocytosis
- 1 December 1973
- journal article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 144 (3) , 1006-1012
- https://doi.org/10.3181/00379727-144-37730
Abstract
A bioassay for the detection of the thrombopoietic stimulating factor (TSF or thrombopoietin) that utilizes thrombo–cythemic mice has been presented. A single injection of anti–mouse platelet serum (AMPS) to mice produced a characteristic thrombocytopenia that was followed by rebound thrombocytosis. Two to three days later (7–8 days after the AMPS injection), a significantly (p < 0.05) depressed thrombo–poiesis existed in response to the thrombocytosis. Normal and thrombocythemic mice were injected with normal sheep sera or sera from platelet–depleted sheep and Na235SO4 incorporation into platelets was measured. A greater difference in 35S uptake existed between AMPS–treated control mice (mice injected with normal sheep serum) and AMPS–treated stimulated mice (mice injected with serum from platelet–depleted sheep) than in normal mice treated with the same sera. Also, TSF in sheep sera was shown to lose activity after storage at −20°.This publication has 0 references indexed in Scilit: