Detection of specific bacteriocin-producing lactic acid bacteria by colony hybridization
Open Access
- 1 June 1998
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Applied Microbiology
- Vol. 84 (6) , 1099-1103
- https://doi.org/10.1046/j.1365-2672.1998.00448.x
Abstract
A colony hybridization method for detecting lactic acid bacteria encoding specific bacteriocins was developed. Specific PCR-generated probes were used to detect colonies of pediocin PA-1, lactococcin A, enterocin AS-48, nisin A and lacticin 481 producing strains. The probes were shown to be sensitive and specific for sequences belonging to the structural genes of the respective bacteriocins.Keywords
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