THE INTERACTION OF POLYSACCHARIDES WITH A SPECTROSCOPIC PROBE: THE ANION EFFECT ON THE BINDING SITE OF HEPARIN
- 22 January 2001
- journal article
- Published by Taylor & Francis in Spectroscopy Letters
- Vol. 34 (1) , 25-34
- https://doi.org/10.1081/sl-100001447
Abstract
Heparin (Hep) has a variety of biological activities, most of them due to Hep's high anionic groups. The average binding number (n) is proposed to express the density of anion's group (binding site) on Hep. To gain insight into the binding site of Hep under the interference of anions, we have developed a method that makes use of isosbestic points in the reaction system in which two reagents compete for the same dye ion, and one complex is colorless. It is also necessary to determine the molar absorptivities of the various species concerned.Keywords
This publication has 19 references indexed in Scilit:
- A Mathematical Model for Interaction of Spectroscopic Probe with PolysaccharidesSpectroscopy Letters, 1998
- Mechanism of Methylene Blue Action and Interference in the Heparin AssaySpectroscopy Letters, 1998
- Mechanism of Interference and Azur A Response in the Heparin AssayAnalytical Letters, 1998
- A Crucial Effect of Ligand Clustering on the Inhibition of Binding by L-Selectin in Intercellular AdhesionBiochemical and Biophysical Research Communications, 1994
- Interaction Between Glycosaminoglycans, Platelets, and LeukocytesSeminars in Thrombosis and Hemostasis, 1994
- Effects of Heparin and Its Desulfated Derivatives on Leukocyte-Endothelial AdhesionSeminars in Thrombosis and Hemostasis, 1994
- Differential binding of chemokines to glycosaminoglycan subpopulationsCurrent Biology, 1994
- Interleukin‐8 is a potent eosinophil chemo‐attractantClinical and Experimental Allergy, 1994
- Synthesis of a N′-Alkylamine Anticoagulant Active Low-Molecular-Mass Heparin for Radioactive and Fluorescent LabelingAnalytical Biochemistry, 1994
- Calcium-Dependent Heparin-Like Ligands For L-Selectin in Nonlymphoid Endothelial CellsScience, 1993