32P-Postlabelling determination of DNA adducts of malonadlehyde in humans: total white blood cells and breast tissue
- 1 August 1995
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 16 (8) , 1847-1851
- https://doi.org/10.1093/carcin/16.8.1847
Abstract
DNA adducts of malonaldehyde (MA) were measured in total white blood cells (TWBC) fo healthy individuals from both sexes and different ages, as well as in breast tissue (BT) samples obtained from, healthy females undergoing reduction mammaplasty. A large interindividual variation in adduct levels was observed. The average adduct level found in TWBC, considering both sexes and all ages was 2.6 ± 1.2 adducts/107 nucleotides (n = 26). A similar average DNA adduct concetratioon was found in BT and amounted to 3.0 ± 1.3 (n = 7) adducts/107 nucleotides. Our results whow that DNA adducts of MA can be measured in humans using 32P-postlabelling in combination with nucleas P1 and reversed-phase HPLC as adduct enrichment procedures, and furher validate these adducts as suitable biomarkers for the measurement of DNA damage inflicted by endogenously induced oxidative processes such as lipd peroxidation.Keywords
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