Cellulase-catalysed glycosylation reactions: simple route towards a highly selective synthesis of oligosaccharides
- 1 January 1994
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of the Chemical Society, Perkin Transactions 1
- No. 13,p. 1851-1857
- https://doi.org/10.1039/p19940001851
Abstract
Cellulase (EC 3.2.1.4) can be used for the enzyme-catalysed synthesis of oligosaccharides in a mixed buffer/organic solvent system. Besides glucopyranosides and cellooligosaccharides, non -glucosidic saccharides and modified glucoses as glycosyl acceptors also react with β-lactosyl fluoride as glycosyl donor. The reaction proceeds in a completely stereo- and regio-specific manner, giving rise to coupling products having β(1→4) glycosidic bonds exclusively. Based on these results, a model for the active site of cellulase (Trichoderma viride) and the orientation of the donor and acceptor therein is proposed.Keywords
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