STUDIES OF THE MECHANISMS FOR THE INDUCTION OF INVIVO TUMOR-IMMUNITY .4. ENHANCEMENT OF THE INVITRO GENERATION OF SECONDARY CELL-MEDIATED CYTOTOXIC RESPONSE BY NORMAL PERITONEAL-MACROPHAGES AND THEIR CULTURE SUPERNATANTS

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 122  (4) , 1519-1527
Abstract
The effect of macrophages on the induction of the cell-mediated cytotoxicity against a leukemia in a syngeneic system was investigated. The addition of exogenous peritoneal cells from normal C57BL/6 mice enhanced the in vitro secondary cell-mediated cytotoxic response of spleen and lymph node cells as responding cells against syngeneic FBL-3 leukemia. Peritoneal phagocytic macrophages seemed responsible for the enhancement. No inhibitory effect was demonstrated by the addition of peritoneal macrophages at a concentration as high as 20%, whereas the primary cytotoxic allograft response was significantly suppressed. There was no absolute restriction of macrophage-T [thymus-derived] cell interaction by an H-2 barrier. Supernatants of peritoneal macrophage cultures enhanced this cell-mediated cytotoxic response. There was no difference between the effects of syngeneic or allogeneic peritoneal macrophage culture supernatants.

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