Degradation of the D1 Protein of Photosystem-II Reaction Centre by Ultraviolet-B Radiation Requires the Presence of Functional Manganese on the Donor Side
- 1 February 1995
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 227 (3) , 723-729
- https://doi.org/10.1111/j.1432-1033.1995.tb20194.x
Abstract
The in vivo effects of ultraviolet-B radiation (280-320 nm) on photosystem-II activity and degradation of the D1 protein are investigated and compared with the in vitro results on isolated thylakoids and other detergent-extracted photosystem-II preparations. A cleavage site in the second transmembrane segment of the D1 protein, giving rise to a 20-kDa C-terminal and a 13-kDa N-terminal fragment pair, is detected after irradiation of entire leaves as well as in all photosystem-II preparations, irrespective of their actual ability to evolve oxygen but depending on the presence of Mn ions associated with the water-splitting system. Damage to the plastoquinone moiety, observed by other authors, is confirmed and is proposed to be responsible for the impairment of electron-transport activity, but not for the observed cleavage of the D1 protein.Keywords
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