Abstract
Two 4-point test crosses comprising 87,000 tomato plants were grown and the data collected from 28 subgroups. Each subgroup consisted of 2000 or 5000 plants and should give a valid estimate of the 3 recombination values. The 28 values for each interval give more outlyers (23% are outside the 95% limits set by the SD calculated by the binomial formula .sqroot..cxa.p.cxa.q/n [where .cxa.p = mean, .cxa.q = 1 - .cxa.p and n = subgroup size]) than would be expected by chance. If each subgroup was regarded as the control and the other groups tested against this, then 42% of the time the 2 subgroups would be significantly different. While the causes of these changes in recombination value are unknown and therefore uncontrollable, they must be anticipated in all such studies. Control and treatment must be replicated enough that chance extreme values will not be attributed to treatment.

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