EPR titration of ovine prostaglandin H synthase with hemin
- 9 January 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 165 (2) , 293-296
- https://doi.org/10.1016/0014-5793(84)80189-1
Abstract
To characterize further the prosthetic group of PGH synthase (EC 1.14.99.1), titrations of the apoenzyme with hemin were investigated by EPR. The first hemin bound per polypeptide showed an EPR signal at g = 6.7 and 5.3 (rhombicity 9%) and was tentatively assigned to the hemin effective as prosthetic group of PGH synthase. Additional hemin bound showed a less rhombic signal (g = 6.3 and 5.8, rhombicity 3%) presumably due to nonspecific hydrophobic binding sites not effective in catalysis.Keywords
This publication has 12 references indexed in Scilit:
- The heme-binding properties of prostaglandin synthetase from sheep vesicular gland.Journal of Biological Chemistry, 1981
- EPR spectra of structurally modified rooflike folded hemins: Axial ligand-induced rhombicityEuropean Biophysics Journal, 1980
- Models for ferric cytochrome P450. Characterization of hemin mercaptide complexes by electronic and ESR spectraJournal of Inorganic Biochemistry, 1979
- On the haemoprotein character of prostaglandin endoperoxide synthetaseBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1979
- Prostaglandins and ThromboxanesAnnual Review of Biochemistry, 1978
- Purification and characterisation of prostaglandin endoperoxide synthetase from sheep vesicular glandsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1977
- Purification of the cyclooxygenase that forms prostaglandins. Demonstration of two forms of iron in the holoenzyme.Journal of Biological Chemistry, 1976
- The Effects of Protein Conformation on the Heme Symmetry in High Spin Ferric Heme Proteins as Studied by Electron Paramagnetic ResonanceJournal of Biological Chemistry, 1971
- The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel ElectrophoresisJournal of Biological Chemistry, 1969
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951