THE EFFECTS OF CHOLINE AND OTHER FACTORS ON THE RELEASE OF ACETYLCHOLINE FROM THE STIMULATED PERFUSED SUPERIOR CERVICAL GANGLION OF THE CAT

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Abstract
When the superior cervical ganglion of the cat was perfused with Locke solution, the amount of acetylcholine released into the perfusate decreased during successive periods of repetitive stimulation of the preganglionic nerve. Addition of choline to the perfusion fluid prevented this decrease. Choline also significantly increased (P>0.01) the initial output of acetylcholine. In contrast, variation of the physostigmine concentration or of the pCO2 and pH of the perfusion fluid had no statistically significant effect (P>0.05) upon the initial release of acetylcholine.

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