The specific incorporation of labelled aromatic amino acids into proteins through growth of bacteria in the presence of glyphosate
- 12 November 2001
- journal article
- Published by Wiley
- Vol. 272 (1-2) , 34-36
- https://doi.org/10.1016/0014-5793(90)80442-l
Abstract
Growth of Escherichia coli in the presence of glyphosate, an inhibitor of aromatic amino acid biosynthesis, has permitted the production of proton translocating ATPase that is specifically labelled with 5-fluorotryptophan. Five sets of 19F nuclear magnetic resonances are resolved. The use of glyphosphate should be of wide applicability in the preparation of proteins labelled in aromatic amino acid residues for NMR studies.Keywords
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