A new method for quantitative determination of protein associated with crustacean chitin.
- 1 January 1987
- journal article
- Published by Japan Society for Bioscience, Biotechnology, and Agrochemistry in Journal of the agricultural chemical society of Japan
- Vol. 61 (4) , 437-441
- https://doi.org/10.1271/nogeikagaku1924.61.437
Abstract
This method is based on hydrolysis of chitin-associated protein with 10 N NaOH in an autoclave at 121°C for 60 minutes and colorimetric determination of the ninhydrin-positive substances in the hydrolysate. In applying this method, a linear relationship was observed between protein content determined gravimetrically and absorbance of the hydrolysate-ninhydrin complex in demineralized cuticles of three prawn species, two crab species and a squilla species.Keywords
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