The Ins(1,4,5)P3 binding site of bovine adrenocortical microsomes: function and regulation
- 1 June 1989
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 260 (2) , 593-596
- https://doi.org/10.1042/bj2600593
Abstract
Adrenocortical microsomes possess a single population of Ins(1,4,5)P3-specific binding sites [IC50 5.9 +/- 0.9 nM; Palmer, Hughes, Lee & Wakelam (1988) Cell. Signalling 1, 147-156]. Competition studies showed that Ins(1:2-cyclic,4,5)P3 exhibits a 21-fold lower affinity for the site than Ins(1,4,5)P3 (IC50 124 +/- 16 nM). The affinity of the binding sites for Ins(1,4,5)P3 was not influenced by the non-hydrolysable GTP analogues GTP gamma S and Gpp[NH]p or by preincubation of the binding protein with a preparation of partially purified protein kinase C in the presence of ATP and TPA (12-O-tetradecanoylphorbol 13-acetate). These observations are discussed with reference to the identify and function of the Ins(1,4,5)P3 binding site.This publication has 32 references indexed in Scilit:
- Stimulation of hepatic inositol 1,4,5-trisphosphate kinase activity by Ca2+-dependent and -independent mechanismsBiochemical Journal, 1988
- Cyclic AMP-dependent phosphorylation of a brain inositol trisphosphate receptor decreases its release of calcium.Proceedings of the National Academy of Sciences, 1988
- Inositol 1:2(cyclic),4,5-trisphosphate is not a major product of inositol phospholipid metabolism in vasopressin-stimulated WRK1 cellsBiochemical Journal, 1988
- Activation of sea urchin eggs by inositol phosphates is independent of external calciumBiochemical Journal, 1988
- Highly Cooperative Opening of Calcium Channels by Inositol 1,4,5-TrisphosphateScience, 1988
- Regulation of inositol 1,4,5-trisphosphate metabolism in insulin-secreting RINm5F cellsBiochemical Journal, 1988
- "Calciosome," a cytoplasmic organelle: the inositol 1,4,5-trisphosphate-sensitive Ca2+ store of nonmuscle cells?Proceedings of the National Academy of Sciences, 1988
- Solubilization, purification, and characterization of an inositol trisphosphate receptor.Journal of Biological Chemistry, 1988
- Protein kinase C-mediated negative-feedback inhibition of unstimulated and bombesin-stimulated polyphosphoinositide hydrolysis in Swiss-mouse 3T3 cellsBiochemical Journal, 1987
- Inositol 1,4,5‐trisphosphate and 5′‐GTP induce calcium release from different intracellular poolsFEBS Letters, 1987