The three-dimensional structure of the flagellar apparatus of Chrysosphaerella brevispina (Chrysophyceae) as viewed by high voltage electron microscopy stereo pairs

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Abstract
Stereo-pair micrographs of thick sections, using high voltage electron microscopy as well as conventional electron microscopy, are used to examine the flagellar apparatus of Chrysosphaerella brevispina, a silica-scaled alga. This follows the general pattern found among the Chrysophyceae, but differs from that found among the silica-scaled Synurophyceae, providing further evidence for the separation of these two classes. A delicate rhizoplast is present in Chrysosphaerella, but it extends towards a mitochondrion and the plasma membrane rather than towards the nucleus. The R, microtubular root forms a broadly sweeping arc with numerous cytoskeletal microtubules spreading out from it. The R2 root consists of two microtubules that pass over the chloroplast on the left side of the cell. The R3 and R4 microtubular roots form antiparallel loops around and under the short flagellum. The R4 root originates on the anterior surface of the short flagellum. The R3 root originates near the axis of the basal bo...

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