COMPARATIVE-STUDY ON LYSOSOMAL ACCUMULATION OF GA-67 AND IN-111 IN MORRIS HEPATOMA 7316A

  • 1 January 1977
    • journal article
    • research article
    • Vol. 18  (8) , 835-839
Abstract
Intracellular localization of 67Ga and 111In was investigated in Morris hepatoma 7316A and normal Buffalo rat liver cells by a cell fractionation method at 48 h after an i.p. injection of the nuclides. Lysosomal fractions of the tumor and normal liver cells had the highest relative specific radioactivities of the nuclides (P < 0.001). In a gradual solubilization experiment, the release of the nuclides at the same time as the acid phosphatase from the lysosomal fractions (P < 0.001) was thought to indicate that lysosomes are the site of accumulation for both nuclides whether in tumor or normal liver cells. Fragility of the tumor lysosomes might be inferred from the significantly greater regression coefficient in relation to the lysosomal fraction of tumor cells than that of normal liver cells when labeled with 67Ga (P < 0.001). The poorer confidence limit for the regression coefficient in relation to the lysosomal fraction of tumor cells labeled with 67Ga seemed to indicate that 67Ga determines lysosomal functions of tumor cells more precisely than 111In in scintiscanning.