HISTOCHEMICAL STUDIES ON GENETICAL CONTROL OF HORMONAL ENZYME INDUCIBILITY IN THE MOUSE II: ESTERASE ISOZYMES OF THE NORMAL EPIDIDYMIS DEMONSTRATED BY SUBSTRATE VARIATION AND BY ENZYME INHIBITORS

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Abstract
Non-specific esterases of the normal mouse epididymis were studied by the .alpha.-naphthyl acetate (.alpha.-NA) and 5-bromoindoxyl acetate (5-BIA) methods and by these techniques in combination with inhibitors. Several esterase isozymes are present in the epididymis. The .alpha.-NA method produces a strong reaction to at least 1 isozyme more than is demonstrable by 5-BIA. The latter technique distinguishes between different cells which show identical reaction to .alpha.-NA. The use of inhibitors confirms these differences. The techniques used enable localization of the cells or origin of various isoenzymes in a complex organ. A basis for studies on the genetical control of cell function is provided.