FACTORS AFFECTING THE INCORPORATION OF PRECURSORS INTO THE PHOSPHOLIPIDS OF EHRLICH ASCITES CELLS

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Abstract
The incorporation of choline-methyl-14C, ethanolamine-1,2-14C, and32Piinto the phospholipids of Ehrlich ascites cells is enhanced under aerobic conditions by the presence of glucose. The extent of incorporation of each of the three precursors is the same under aerobic or anaerobic conditions if glucose is present. The formation of free phosphocholine and free phosphoethanolamine exceeds by a factor of 10 the incorporation of choline-methyl-14C and ethanolamine-1,2-14C into the phospholipids. The data suggest that acid-soluble phosphocholine and phospho-ethanolamine are not in rapid isotopic equilibrium with the phosphorylated bases present at the sites of phospholipid synthesis.