Pharmacological modulation of the contractile response to toluene diisocyanate in the rat isolated urinary bladder

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Abstract
1 Toluene diisocyanate produced concentration-dependent contractions of the rat isolated urinary bladder. 2 The contractions were tetrodotoxin-resistant and were abolished by previous exposure of the strips to capsaicin. 3 Indomethacin (5 μm) and ruthenium red (30 μm) inhibited toluene diisocyanate-induced contractions. Responses expressed as a percentage of the response obtained with substance P, 30 nm, were respectively 141.6 ± 24.8% and 20.1 ± 5.1% in control and indomethacin-treated strips (P < 0.005); 123.0 ± 30.2% and 14.0 ± 6.5% in control and ruthenium red-treated strips (0.01 < P < 0.05). 4 These results suggest that toluene diisocyanate-induced contractions of the rat isolated bladder are the result of the release of cyclo-oxygenase products which may act by activating the capsaicin receptor.