Anticomplementary nature of smaller DNA produced during synthesis of extensive DNA copies of poliovirus RNA.
- 1 October 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (10) , 3408-3412
- https://doi.org/10.1073/pnas.73.10.3408
Abstract
The reverse transcriptase (RNA-directed DNA nucleotidyltransferase) from avian myeloblastosis virus is able to make an extensive, possibly complete, complementary DNA copy of intact poliovirus RNA. In the presence of high concentrations of deoxyribonucleoside triphosphates, ribonucleoside triphosphates, or sodium pyrophosphate, this DNA is the only species produced. Without these additives, however, a second size class of DNA is also synthesized. This material has a sedimentation coefficient between roughly 4 and 10 S and is produced later in the reaction, largely after synthesis of the larger complementary DNA has ceased. The smaller DNA consists primarily of material anticomplementary to the RNA template and contains a faithful and uniform representation of the viral sequences. It most likely arises by transcription of the larger DNA species.This publication has 3 references indexed in Scilit:
- Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities.Proceedings of the National Academy of Sciences, 1976
- Conditions for the selective synthesis of DNA complementary to template RNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973