Oxidative Phosphorylation Coupled with Nitrate RespirationII. Phosphorylation Coupled with Anaerobic Nitrate Reduction in a Cell-Free Extract of Escherichia coli

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Abstract
Oxidative phosphorylation coupled with anaerobic nitrate reduction occurs in cell-free extracts of E. coli. The P/NO3- ratios during the reduction of nitrate to nitrite were 0. 65 and 1.1 with glutamate and citrate, respectively, as electron donors. Phosphorylation coupled with nitratreduction was inhibited in the presence of KCN and 2,4-dinitrophenol but not in the presence of CO. Antimycin-A and amytal acted as uncouplers of phosphorylation coupled with nitrate reduction.